Surging toxicity studies, drug discovery projects, and other research ventures have driven the demand for enhanced imaging over the past few years. This is expected to sustain the demand for the high content screening (HCS) technique & instrument in the near future. Persistence Market Research in its study reveals that, the global high content screening market is projected to witness an impressive 6.1% CAGR through 2025.
Factors Driving the Growth of Global High Content Screening Market Include
- Numerous life science research activities along with increasing government support
- Research-based biotechnology and pharmaceutical companies, especially in developing regions
- Growing emphasis on secondary screening of cells for the purpose of toxicity studies
- Assessment of risks related to chronic diseases which employ drug discovery processes through high content screening
- Automated systems’ development for cell separation & scalability
- Soaring demand for therapeutic cell purification protocols that are highly efficient
HCS to Bridge the Gap between Throughput and Depth of Biological Experiments
High content screening, an effective technology, is designed for providing digital cellular imaging with an ability for investigation of multiple phenotypic profiling. Essential fields such as pharmaceutical industry, biochemistry and cellular biology are being influenced owing to the development this automated microscopy approach with unique analytical platform. This paradigm is expected to bridge the gap between throughput and depth of biological experiments, allowing better cellular complex behavior & response interpretation. Such an automated multi-parametric technology renders unbelievable endeavor in analysis and evaluation of large amount of data by multiple or single images.
Various applications have been analyzed by using HCS & cellular imaging technology. These include cell cycle, cell differentiation, cell proliferation, cytoskeletal rearrangement, protein expression, neurite outgrowth, apoptosis, cell migration, cell shape changes, cytotoxicity and signaling pathways. Impact of high content screening on stem cells offers evaluation of complex signaling pathways, to regulate stem cell development, differentiation and self-renewal. HCS approaches utilized for investigating cellular signaling pathways contributes in the determination of disease-related functional properties comprised by small molecules.
HCS to Examine Signaling between Cancer Cells & their Microenvironment
The team leader at ICR (The Institute of Cancer Research) in London, Dr. Chris Bakal, stated the benefits of utilizing high content screening for functional genomics and phenotypic profiling at the Labcyte Genomics Symposium. HCS can be utilized for examination of signaling between cancer cells and their microenvironment. Cancer cells exhibit various shape changes to metastasize. Majority of the cancer patients die owing to metastasis. New therapeutic avenues maybe provided through understanding the ways of cells changing shapes.
High Content Screening Approach Reveals key roles for Rab33b, OATL1 and Myo6
Synthetic nanoparticles form indispensable tools for drug delivery and imaging. However full characterization of molecular details of cellular trafficking & internalization is still in progress. According to current knowledge, most of the nanoparticles, following endocytosis, pass from endosomes to lysosomes. To comprehensively understand nanoparticle uptake & trafficking, it is fundamental to design effective strategies for drug delivery that can use bypass lysosomal accumulation or endocytic pathway. Application of RNA interference-based HCS microscopy strategy will help accessing intracellular trafficking of polystyrene nanoparticles (fluorescently-labeled) in HeLa cells. Screening a total of 408 genes, involved in cytoskeleton & membrane function revealed roles for Rab33b, myosin VI and OATL1. This provided systematic quantitative large-scale assessment of proteins responsible for nanoparticle trafficking and paved a way for succeeding genome-wide studies.
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